中国呼吸与危重监护杂志

中国呼吸与危重监护杂志

应用二代测序技术诊断肺结核病及肺非结核分枝杆菌病各一例并文献复习

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目的利用二代基因测序(NGS)技术对 2 例可疑肺结核病及肺非结核分枝杆菌病的肺部感染患者进行明确诊断,同时进行文献复习,探讨 NGS 技术在病原微生物感染诊断方面的价值。方法收集 2 例拟诊肺部感染待查患者的支气管肺泡灌洗液,采用 NGS 技术检测致病病原体基因。以 “二代测序 ”或 “NGS ”和 “微生物 ”或 “感染 ”为关键词,通过万方和中国知网数据库对 2000 年 1 月至 2018 年 1 月的中文文献进行检索,以 “NGS,infectious diseases,China ”为关键词,通过 PubMed 数据库检索 2018 年 1 月前的英文文献,结合文献进行分析。结果通过 NGS 分别检测到结核分枝杆菌 1 例及非结核分枝杆菌 1 例。共检索到中文文献 221 篇,排除学位论文、会议论文和报纸,最终保留 “感染性疾病及传染病 ”和 “呼吸系统疾病 ”学科分组中的期刊报道 10 篇,英文文献 3 篇。报道中均提出 NGS 对相关病原体的诊断与研究作用。结论NGS 有望实现精准医疗的目的,如感染性疾病的早期诊断、控制传播、精准治疗、良好预后等。

ObjectiveTo explore application value of next-generation sequencing (NGS) technology in diagnosis of pathogenic microorganism infection through two cases report and literature review.MethodsThe NGS technology was used to make clear diagnosis of two cases of suspected pulmonary tuberculosis and pulmonary nontuberculous mycobacterial diseases. Bronchoalveolar lavage fluid of these two patients was collected for gene detection of pathogens using the NGS technology. A systematic literature review was performed for similar published cases in WanFang and CNKI database, using the keywords (next-generation sequencing) OR (NGS) AND (microorganism OR infection) from January 2000 to January 2018, using the PubMed database to retrieve the English literature before January 2018 with the " NGS, infectious diseases, China” as keywords.ResultsOne case of Mycobacterium tuberculosis and one case of non-tuberculous Mycobacteria were detected respectively. A total of 221 Chinese literatures and 3 English literatures were retrieved, excluding dissertations, conferences and newspapers. Finally, 10 articles were published in the infectious diseases and respiratory diseases subjects. The role of NGS technology in the diagnosis and study of related pathogens is proposed.ConclusionThe NGS method is expected to achieve precision medical purposes, such as early diagnosis of infectious diseases, transmission control, accurate treatment, good prognosis and so on.

关键词: 结核分枝杆菌; 非结核分枝杆菌; 感染; 二代基因测序

Key words: Mycobacterium tuberculosis; Non-tuberculous mycobacteria; Infection; Next-generation sequencing

引用本文: 黄畅宇, 胡瑞成, 张有志, 李圣青. 应用二代测序技术诊断肺结核病及肺非结核分枝杆菌病各一例并文献复习. 中国呼吸与危重监护杂志, 2019, 18(1): 21-25. doi: 10.7507/1671-6205.201803032 复制

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