中国呼吸与危重监护杂志

中国呼吸与危重监护杂志

骨膜蛋白在皮肌炎和类风湿性关节炎伴间质性肺疾病患者支气管肺泡灌洗液中的检测及临床意义

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目的检测皮肌炎(DM)和类风湿性关节炎(RA)伴间质性肺疾病(ILD)患者支气管肺泡灌洗液(BALF)中骨膜蛋白的表达及临床意义。方法对 44 例 DM-ILD 和 28 例 RA-ILD 患者进行支气管肺泡灌洗,回收 BALF,检测 BALF 中骨膜蛋白和涎液化糖链抗原-6(KL-6)水平,并对 BALF 进行细胞分类计数。对每位患者进行高分辨率 CT 评分。另选 30 例无 ILD 病变的患者作为对照组。结果DM-ILD 和 RA-ILD 患者 BLAF 中骨膜蛋白、KL-6 水平均明显高于对照组(均 P<0.05)。骨膜蛋白水平与 BALF 中淋巴细胞计数、KL-6 水平均呈正相关(均P<0.05)。骨膜蛋白与 DM-ILD、RA-ILD 患者高分辨率 CT 评分呈正相关(P<0.05)。结论DM-ILD 和 RA-ILD 患者 BALF 中骨膜蛋白水平升高,是 CTD-ILD 的潜在预测指标。

ObjectiveTo explore the expression of periostin in bronchoalveolar lavage fluid (BALF) of patients with dermatomyositis-related interstitial lung disease (DM-ILD) and rheumatoid arthritis-related interstitial lung disease (RA-ILD).MethodsA total of 44 patients with DM-ILD and 28 patients with RA-ILD were underwent bronchoalveolar lavage. Cells in BALF were collected and analyzed by absolute different cell counts. The level of periostin and Krebs von den Lungen-6 (KL-6) were tested by enzyme linked immunosorbent assay. Results of high resolution CT of patients were scored. Thirty patients without interstitial lung disease (ILD) served as a control group.ResultsLevels of periostin and KL-6 were significantly increased in BALF of patients with DM-ILD and RA-ILD compared with control group (all P<0.05). Levels of periostin were positively correlated with lymphocyte counts and levels of KL-6 in BALF (allP<0.05). Furthermore, levels of periostin were significantly correlated with high resolution CT scores (P<0.05).ConclusionsLevels of periostin are increased in patients with DM-ILD and RA-ILD. Periostin might be served as an indicator of CTD-ILD.

关键词: 结缔组织病间质性肺疾病; 支气管肺泡灌洗液; 骨膜蛋白; 涎液化糖链抗原-6

Key words: Connective tissue disease-related interstitial lung disease; Bronchoalveolar lavage fluid; Periostin; Krebs von den Lungen-6

引用本文: 贺新伟, 骆琼珍, 尚颖, 高占成. 骨膜蛋白在皮肌炎和类风湿性关节炎伴间质性肺疾病患者支气管肺泡灌洗液中的检测及临床意义. 中国呼吸与危重监护杂志, 2018, 17(5): 465-469. doi: 10.7507/1671-6205.201802025 复制

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