中国呼吸与危重监护杂志

中国呼吸与危重监护杂志

沉默信息调节因子 2 相关酶 1 在慢性阻塞性肺疾病患者血清中的表达水平及临床意义

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目的探讨沉默信息调节因子 2 相关酶 1(SIRT1)在慢性阻塞性肺疾病(简称慢阻肺)患者血清中的表达水平及临床意义。方法选取本院慢阻肺急性加重期患者 40 例、慢阻肺稳定期患者 30 例作为研究组,另选体检健康者 20 例作为对照组。对所有受试者进行肺功能检查。应用酶联免疫吸附法和蛋白免疫印迹检测血清 SIRT1、核因子-κB(NF-κB)、基质金属蛋白酶-9(MMP-9) 水平。结果与对照组相比,慢阻肺稳定期组和慢阻肺急性加重组患者第 1 秒用力呼气容积占预计值百分比(FEV1%pred)及第 1 秒用力呼吸容积占用力肺活量百分比(FEV1/FVC)均显著降低(均 P<0.05)。与慢阻肺稳定期组相比,慢阻肺急性加重组患者 FEV1%pred 及 FEV1/FVC 均显著降低(P<0.05)。与对照组相比,慢阻肺稳定期组和慢阻肺急性加重组患者血清 SIRT1 水平均显著降低,而 NF-κB 和 MMP-9 水平均显著增加(P<0.05)。与慢阻肺稳定期组患者相比,慢阻肺急性加重组患者血清 SIRT1 水平显著下降,而 NF-κB 和 MMP-9 水平均显著增加(均P<0.05)。经 Pearson 直线相关法分析,血清 SIRT1 水平与慢阻肺患者 FEV1%pred(P<0.05)、FEV1/FVC(P<0.05)等肺功能指标呈显著正相关。血清 SIRT1 水平与 NF-κB(P<0.05)、MMP-9(P<0.05)蛋白表达水平呈显著负相关。结论慢阻肺患者血清 SIRT1 水平降低与肺功能下降相关,有可能作为慢阻肺的潜在生物标志物。

ObjectiveTo investigate the expression of SIR2-related enzyme 1 (SIRT1) in serum of patients with chronic obstructive pulmonary disease (COPD) and its clinical significance.MethodsForty patients with acute exacerbation of COPD (acute-COPD group) and 30 patients with stable COPD (stable-COPD group) were selected as study groups. Twenty healthy subjects were recruited as a control group. Serum SIRT1, nuclear factor (NF) -κB, and matrix metalloproteinases (MMP) -9 levels were detected by enzyme-linked immunosorbent assay and Western blot.ResultsCompared with the control group, the FEV1%pred and FEV1/FVC were significantly decreased in the acute COPD patients and the stable COPD patients (P<0.05). Compared with the stable-COPD group, FEV1%pred and FEV1/FVC were significantly decreased in the acute-COPD group (P<0.05). Compared with control group, the serum SIRT1 in the stable-COPD group and the acute-COPD group were significantly decreased, while NF-κB and MMP-9 were significantly increased (P<0.05). Compared with the stable-COPD group, SIRT1 in the acute-COPD group was significantly decreased, while NF-κB and MMP-9 were significantly increased (P<0.05). SIRT1 were positively correlated with FEV1%pred (P<0.05) and FEV1/FVC (P<0.05) in the COPD patients by Pearson linear correlation analysis. SIRT1 was negatively correlated with NF-κB (P<0.05) and MMP-9 (P<0.05).ConclusionIn COPD patients, decreased levels of serum SIRT1 is associates with decreased lung function, which may be used as a potential biomarker of COPD.

关键词: 沉默信息调节因子 2 相关酶 1; 慢性阻塞性肺疾病; 肺功能; 血清

Key words: SIR2-related enzyme 1; Chronic obstructive pulmonary disease; Pulmonary function; Serum

引用本文: 郭国华, 肖建宏, 宋彬, 曾林淼. 沉默信息调节因子 2 相关酶 1 在慢性阻塞性肺疾病患者血清中的表达水平及临床意义. 中国呼吸与危重监护杂志, 2018, 17(4): 336-340. doi: 10.7507/1671-6205.201706042 复制

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