中国呼吸与危重监护杂志

中国呼吸与危重监护杂志

哮喘患者外周血程序性细胞死亡因子-1 的表达研究

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目的探讨哮喘患者外周血血清程序性细胞死亡因子-1(PD-1)、γ 干扰素(IFN-γ)、白细胞介素-4(IL-4)及辅助性 T 细胞(Th 细胞)的表达。方法以江苏省连云港市第一人民医院呼吸内科收治的 41 例哮喘患者为研究对象,依据病情分为急性发作组(20 例)和慢性持续组(21 例),另选 18 例健康者作为对照组。患者在起病入院时抽取外周血,采用酶联免疫吸附法检测 PD-1、IFN-γ、IL-4 的浓度,流式细胞仪检测外周血 Th1、Th2 及 Th1/Th2 的表达。结果与健康对照组比较,慢性持续组、急性发作组外周血 PD-1、IFN-γ 浓度降低,IL-4 浓度升高,Th1 细胞比率、Th1/Th2 比值降低,Th2 细胞比率升高,差异均有统计学意义(均 P<0.05)。与慢性持续组比较,急性发作组外周血 PD-1、IFN-γ 浓度更低,IL-4 浓度更高,Th1 细胞比率、Th1/Th2 比值更低,Th2 细胞比率更高,差异均有统计学意义(均P<0.05);急性发作组 PD-1 与 IFN-γ、Th1 呈正相关(r 值分别为 0.678 和 0.712,均 P<0.05),与 IL-4、Th2 呈负相关(r 值分别为 –0.745 和 –0.700,均 P<0.05)。结论哮喘患者外周血 PD-1 降低,急性发作组更低,且与常规的哮喘检测指标呈相关性。

ObjectiveTo investigate the expression of programmed cell death-1 (PD-1), interferon-γ (IFN-γ), interleukin-4 (IL-4) and T help cells (Th) in peripheral blood of patients with asthma.MethodsForty-one asthma patients were divided into an acute group (20 cases) and a chronic group (21 cases) according to the disease state of asthma. Another 18 healthy subjects were recruited as a control group. Peripheral blood samples were collected from all the subjects at admission or inclusion. The concentrations of PD-1, IFN-γ and IL-4 were detected by enzyme-linked immunoassay method. The expressions of Th1 and Th2 were detected by flow cytometry method.ResultsThe PD-1 and IFN-γ concentrations, Th1 proportion and Th1/Th2 ratio in the two asthma groups were reduced compared with the healthy control group, and were significantly lower in the acute group compared with the chronic group (all P<0.05). The IL-4 level and Th2 proportion in the two asthma groups were increased compared with the healthy control group, and were significantly higher in the acute group than the chronic group (allP<0.05). In the acute group, the PD-1 level was positively correlated with IFN-γ and Th1 level (r values were 0.678 and 0.712, respectively), and negatively correlated with IL-4 and Th2 (r values were –0.745 and –0.700, respectively).ConclusionThe concentration of PD-1 in patients with asthma is reduced especially in acute asthma, and shows close correlation with conventional index of asthma.

关键词: 哮喘; 程序性细胞死亡因子-1; γ 干扰素; 白细胞介素-4; 辅助性T细胞

Key words: Asthma; Programmed cell death-1; Interferon-γ; Interleukin-4; T help cells

引用本文: 张趁英, 吴兴萍, 李春华, 李家树. 哮喘患者外周血程序性细胞死亡因子-1 的表达研究. 中国呼吸与危重监护杂志, 2018, 17(4): 390-393. doi: 10.7507/1671-6205.201706008 复制

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